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Rabbit抗原名称
CD300LD分子别名
CMRF35-like molecule 5; CLM-5; CD300 antigen-like family member D; CMRF35-A4; CD300d; CMRF35A4免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q6UXZ3克隆号
S-1578-29抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
CD300LD is a member of the CD300 family of proteins and is a single transmembrane protein specifically highly expressed in neutrophils and polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs). It plays a significant role in tumor progression by regulating the recruitment of PMN-MDSCs and inhibiting T-cell infiltration through the STAT3-S100A8/A9 axis. Studies have shown that CD300LD knockout mice exhibit significantly inhibited tumor development, with reduced accumulation of PMN-MDSCs and increased numbers of immune effector cells such as CD4+ and CD8+ T cells and natural killer cells. This suggests that targeting CD300LD may remodel the tumor immune microenvironment from an immunosuppressive state to an activated state, offering potential for personalized targeted therapy. Additionally, CD300LD has been implicated in the regulation of interleukin-6 and tumor necrosis factor production, further highlighting its importance in immune responses.
免疫印迹
WB result of CD300LD Recombinant Rabbit mAb
Primary antibody: CD300LD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
Lane 2: 293T transfected with CD300LD-Myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23, 26 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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