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宿主来源
Rabbit抗原名称
OGN分子别名
Mimecan; Osteoglycin; Osteoinductive factor (OIF); OIF; SLRR3A免疫原
Synthetic Peptide细胞定位
Secreted, Extracellular spaceAccession
P20774克隆号
S-245-327抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
MCF7, HeLa, mouse skeletal muscle, mouse blood vessel, mouse skin, rat skeletal muscle, rat blood vessel, rat skin预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt
OGN (Osteoglycin), also known as Mimecan or SLRR3A, is a member of the small leucine-rich proteoglycan (SLRP) family and a glycosylated protein involved in the development of the extracellular matrix (ECM). It functions as a growth factor that induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2 and may regulate osteoblast differentiation. OGN is expressed in various tissues and is implicated in multiple biological processes, including fibrosis, tumorigenesis, and cardiovascular events. High expression of OGN has been associated with elevated heart left ventricular mass and may play a role in the progression of certain cancers, such as pancreatic cancer, by targeting ID4. Additionally, OGN is detected in blood through mass spectrometry and proximity extension assays, indicating its potential as a biomarker in diseases like pediatric retinoblastoma.
免疫印迹
WB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40 kDaWB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse blood vessel lysate 20 µg
Lane 3: mouse skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40, 25 kDaWB result of OGN Recombinant Rabbit mAb
Primary antibody: OGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat blood vessel lysate 20 µg
Lane 3: rat skin lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 40, 25 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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