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CD28 Recombinant Rabbit mAb (S-1815-10)

T-cell-specific surface glycoprotein CD28,TP44

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1344
规格
数量
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产品介绍

产品介绍 FAQs 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD28

  • 分子别名

    T-cell-specific surface glycoprotein CD28; TP44

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P10747

  • 克隆号

    S-1815-10

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms

  • 阳性样本

    Jurkat, EL4.IL2

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms
    ICC 1:50-1:500 Hu, Ms
背景介绍

  • CD28 is a key costimulatory receptor predominantly expressed on T cells, which plays an essential role in T cell activation, proliferation, and survival. Upon binding to its ligands CD80 (B7-1) and CD86 (B7-2) expressed on antigen-presenting cells, CD28 provides a secondary signal that amplifies the initial T cell receptor engagement with the antigen. This costimulation is crucial for preventing T cell anergy and for the full execution of immune responses, including the production of cytokines and the development of memory T cells. CD28 also contributes to the induction and maintenance of regulatory T cells, and its signaling involves the activation of multiple downstream pathways, including the PI3K-AKT axis, which is important for cell metabolism and survival.

  • 免疫印迹

    • WB result of CD28 Recombinant Rabbit mAb
      Primary antibody: CD28 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: THP-1 whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg
      Lane 3: EL4.IL2 whole cell lysate 20 µg
      Negative control: THP-1 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 25 kDa
      Observed MW: 12, 35~50 kDa

  • 免疫细胞化学

    • ICC shows positive staining in Jurkat cells. Anti-CD28 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in EL4.IL-2 cells. Anti-CD28 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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