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宿主来源
Rabbit抗原名称
Caspase-9分子别名
CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3 (APAF-3); ICE-like apoptotic protease 6 (ICE-LAP6); CASP9; MCH6免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P55211克隆号
S-789-172抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Jurkat, HepG2, HeLa纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu
Caspase-9 is a key initiator caspase in the intrinsic apoptotic pathway, activated by the formation of the apoptosome complex in response to cellular stress such as DNA damage or mitochondrial dysfunction. It is synthesized as an inactive zymogen and becomes active upon recruitment by the apoptosome, which includes cytochrome c and apoptotic protease activating factor-1 (Apaf-1). Once activated, Caspase-9 cleaves and activates downstream effector caspases like Caspase-3, -6, and -7, ultimately leading to apoptosis. Caspase-9 is essential for maintaining cellular homeostasis and preventing the development of diseases such as cancer. Its dysregulation has been implicated in conditions like neurodegenerative disorders and tumorigenesis, making it a potential target for therapeutic interventions.
免疫印迹
WB result of Caspase-9 Recombinant Rabbit mAb
Primary antibody: Caspase-9 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 1 µM Staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 46 kDa
Observed MW: 40, 37, 30 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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