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宿主来源
Rabbit抗原名称
ATP5A分子别名
ATP synthase subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP5F1A; ATP5A1; ATP5AL2; ATPM免疫原
Synthetic Peptide细胞定位
Mitochondrion, Cell membraneAccession
P25705克隆号
S-1542-211抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, Jurkat, A549, Neruo-2a, PC-12预测反应种属
(反应种属缩写表)Or, Pg, Bv, Fr, Ys, Nm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:1000 Hu
ATP5A (ATP synthase F1 subunit alpha) is a key component of the mitochondrial ATP synthase complex, also known as Complex V or F1F0 ATP synthase, which is responsible for ATP production through oxidative phosphorylation. This enzyme complex consists of two major domains: the F1 catalytic core and the Fo membrane proton channel. ATP5A encodes the alpha subunit of the F1 domain, which is essential for ATP synthesis. It is ubiquitously expressed in various tissues, including the heart, kidney, and nervous system. In addition to its role in energy metabolism, ATP5A has been implicated in cancer progression. For example, it interacts with MTA1 (metastasis-associated protein 1) to drive mitochondrial metabolic reprogramming and enhance oxidative phosphorylation, thereby promoting liver metastasis in colorectal cancer. Overexpression of ATP5A has also been observed in metastatic lung adenocarcinomas, highlighting its potential role in tumor development. Defects in ATP5A1 have been linked to severe mitochondrial disorders, such as neonatal mitochondrial encephalopathy. Given its critical function in ATP synthesis and involvement in disease processes, ATP5A1 is an important target for understanding mitochondrial dysfunction and developing therapeutic strategies.
免疫印迹
WB result of ATP5A Recombinant Rabbit mAb
Primary antibody: ATP5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 55 kDaWB result of ATP5A Recombinant Rabbit mAb
Primary antibody: ATP5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 55 kDaWB result of ATP5A Recombinant Rabbit mAb
Primary antibody: ATP5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human liver. Anti-ATP5A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATP5A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-ATP5A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-ATP5A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ATP5A in tumor tissue.
Expression of ATP5A in human tissue.
Expression of ATP5A in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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