Nanog Recombinant Rabbit mAb (S-1823-8)

Homeobox protein NANOG,Homeobox transcription factor Nanog (hNanog)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1337

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Nanog
分子别名

Homeobox protein NANOG; Homeobox transcription factor Nanog (hNanog)
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q9H9S0
克隆号

S-1823-8
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

NCCIT
预测反应种属
(反应种属缩写表)

Cz, CyMk
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

IHC-P ?

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IP 1:50 Hu
IHC-P 1:1000 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IP	1:50	Hu
IHC-P	1:1000	Hu
ChIP	1:20-1:50	Hu

背景介绍

Nanog protein is a key transcription factor crucial for maintaining pluripotency in mammalian early embryos and pluripotent stem cells. It contains a homeodomain and is involved in various cellular processes, including self-renewal and reprogramming. Nanog is essential for the acquisition of pluripotency and the reactivation of the X chromosome, playing a central role in the transcriptional network of pluripotency. It forms autoregulatory loops to maintain embryonic stem cell (ESC) identity and is indispensable for the transition to full pluripotency during reprogramming. Nanog's expression is complex and regulated at multiple levels, and it is associated with diseases such as teratocarcinoma and germ cell and embryonal cancer. Additionally, Nanog has been implicated in cancer progression, acting as a cancer stem cell marker and promoting the self-renewal and long-term proliferative potential of stem cells.

免疫印迹
- WB result of Nanog Recombinant Rabbit mAb
  Primary antibody: Nanog Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: NCCIT whole cell lysate 20 µg
  Negative control: HeLa whole cell lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 35 kDa
  Observed MW: 38 kDa
免疫沉淀
- Nanog Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Nanog in 0.4 mg NCCIT whole cell lysate.
  Western blot was performed on the immunoprecipitate using Nanog Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: NCCIT whole cell lysate 40 µg (Input)
  Lane 2: Nanog Recombinant Rabbit mAb IP in NCCIT whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in NCCIT whole cell lysate
  Predicted MW: 35 kDa
  Observed MW: 38 kDa
  Exposure time: 180 s
免疫组化
- IHC shows positive staining in paraffin-embedded human seminoma. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on NCCIT cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
  Parallel reactions used Nanog Recombinant Rabbit mAb (S-1823-8) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation. Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of Oct-4 and SAT-α in Nanog Recombinant Rabbit
  mAb (S-1823-8)-immunoprecipitated sample.
组织表达图谱
- Expression of Nanog in tumor tissue.
- Expression of Nanog in human tissue.