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宿主来源
Rabbit抗原名称
Nanog分子别名
Homeobox protein NANOG; Homeobox transcription factor Nanog (hNanog)免疫原
Synthetic Peptide细胞定位
NucleusAccession
Q9H9S0克隆号
S-1823-8抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IP反应种属 ?
Hu阳性样本
NCCIT预测反应种属
(反应种属缩写表)Cz, CyMk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
| IP | 1:50 | Hu |
| IHC-P | 1:1000 | Hu |
Nanog protein is a key transcription factor crucial for maintaining pluripotency in mammalian early embryos and pluripotent stem cells. It contains a homeodomain and is involved in various cellular processes, including self-renewal and reprogramming. Nanog is essential for the acquisition of pluripotency and the reactivation of the X chromosome, playing a central role in the transcriptional network of pluripotency. It forms autoregulatory loops to maintain embryonic stem cell (ESC) identity and is indispensable for the transition to full pluripotency during reprogramming. Nanog's expression is complex and regulated at multiple levels, and it is associated with diseases such as teratocarcinoma and germ cell and embryonal cancer. Additionally, Nanog has been implicated in cancer progression, acting as a cancer stem cell marker and promoting the self-renewal and long-term proliferative potential of stem cells.
免疫印迹
WB result of Nanog Recombinant Rabbit mAb
Primary antibody: Nanog Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NCCIT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 35 kDa
Observed MW: 38 kDa
免疫沉淀
Nanog Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Nanog in 0.4 mg NCCIT whole cell lysate.
Western blot was performed on the immunoprecipitate using Nanog Recombinant Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: NCCIT whole cell lysate 40 µg (Input)
Lane 2: Nanog Recombinant Rabbit mAb IP in NCCIT whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in NCCIT whole cell lysate
Predicted MW: 35 kDa
Observed MW: 38 kDa
Exposure time: 180 s
免疫组化
IHC shows positive staining in paraffin-embedded human seminoma. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Nanog antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Nanog in tumor tissue.
Expression of Nanog in human tissue.
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