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CENPM Recombinant Rabbit mAb (S-1870-36)

Centromere protein M,CENP-M,Interphase centromere complex protein 39,Proliferation-associated nuclear element protein 1,C22orf18,ICEN39,PANE1

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1332

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

CENPM
分子别名

Centromere protein M; CENP-M; Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1; C22orf18; ICEN39; PANE1
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

Q9NSP4
克隆号

S-1870-36
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

Ramos, HeLa, PANC-1, HepG2, RAW264.7, C6
预测反应种属
(反应种属缩写表)

AfGrMk, Bb, Cz, Gor, Mq, Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

CENPM protein, also known as centromere protein M, is an inner protein of the kinetochore, a multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help maintain centromere identity at each cell division cycle. CENPM is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation affecting its interaction with CENP-I impairs kinetochore assembly and chromosome alignment, suggesting its requirement for kinetochore formation. CENPM is upregulated in various types of cancer, including glioblastoma multiforme, and is associated with poor prognosis. It plays a role in cell cycle regulation and may contribute to tumor metastasis and recurrence. CENPM is rarely mutated in human cancers, making it a potential prognostic marker and therapeutic target.

免疫印迹
- WB result of CENPM Recombinant Rabbit mAb
  Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Ramos whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: PANC-1 whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 20 kDa
  Observed MW: 23 kDa
- WB result of CENPM Recombinant Rabbit mAb
  Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 20 kDa
  Observed MW: 23 kDa
- WB result of CENPM Recombinant Rabbit mAb
  Primary antibody: CENPM Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 20 kDa
  Observed MW: 23 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti-CENPM antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).