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CDK9 Rabbit Polyclonal Antibody

Cyclin-dependent kinase 9,C-2K,Cell division cycle 2-like protein kinase 4,Cell division protein kinase 9,Serine/threonine-protein kinase PITALRE,Tat-associated kinase complex catalytic subunit,CDC2L4,TAK

Datasheet COA

价格￥400.00 （供应商现货： 3-5个工作日）

货号 S0B1331

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

CDK9
分子别名

Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; CDC2L4; TAK
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P50750
抗体类型

Polyclonal antibody
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HT-29, HeLa, HL-60, 293T, NIH/3T3, RAW264.7, mouse, mouse liver, PC-12, C6, rat testis
预测反应种属
(反应种属缩写表)

Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt

背景介绍

CDK9 protein, located at 9q34.11 and consisting of 372 amino acids, is a CDC2-related kinase and the catalytic subunit of the positive-transcription elongation factor b (P-TEFb). It was first identified in 1994 and is primarily involved in regulating the elongation phase of transcription by RNA polymerase II (RNAPII). CDK9 forms complexes with cyclin T1, T2a, T2b, or K, and these complexes phosphorylate the C-terminal domain (CTD) of RNAPII and negative-transcription elongation factors, thereby overcoming promoter-proximal pausing and facilitating transcription elongation. CDK9 is also involved in transcription initiation and termination, and its activity is regulated by post-translational modifications such as phosphorylation and acetylation. Additionally, CDK9 has been implicated in various cellular processes, including immune responses and metabolic regulation, and is a potential therapeutic target in cancer and other diseases.

免疫印迹
- WB result of CDK9 Rabbit pAb
  Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
  Lane 1: HT-29 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Lane 3: HL-60 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43, 53 kDa
  Observed MW: 38, 50 kDa
- WB result of CDK9 Rabbit pAb
  Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Lane 3: mouse testis lysate 20 µg
  Lane 4: mouse liver lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43, 53 kDa
  Observed MW: 38, 50 kDa
- WB result of CDK9 Rabbit pAb
  Primary antibody: CDK9 Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Lane 3: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43, 53 kDa
  Observed MW: 38, 50 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-CDK9 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.