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SIRT1 Recombinant Rabbit mAb (S-1886-15)

NAD-dependent protein deacetylase sirtuin-1,hSIRT1,NAD-dependent protein deacylase sirtuin-1,Regulatory protein SIR2 homolog 1,SIR2-like protein 1 (hSIR2),SIR2L1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1328

规格

25μl100μl1ml

数量

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

SIRT1
分子别名

NAD-dependent protein deacetylase sirtuin-1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); SIR2L1
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q96EB6
克隆号

S-1886-15
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HEK-293, HeLa, NIH/3T3, mouse testis, C6, rat testis
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:500 Hu, Ms, Rt
ICC 1:500 Ms

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:500	Hu, Ms, Rt
ICC	1:500	Ms

背景介绍

SIRT1 protein, also known as Silent Information Regulator 1, is a NAD+-dependent deacetylase that plays a crucial role in various cellular processes. It is involved in the regulation of gene expression by histone deacetylation and modulates the acetylation levels of numerous substrates, including p53, PGC-1α, NF-κB, FOXO, NCoR, and p300. SIRT1 is essential for cell cycle control, energy metabolism, response to oxidative stress, cell apoptosis, and aging. Calorie restriction (CR) has been shown to increase SIRT1 expression, which is linked to extended lifespan in mammals. SIRT1 also has anti-inflammatory and anti-aging properties, and its dysregulation is associated with diseases such as mitochondrial complex I deficiency and aging. In the brain, SIRT1 upregulates tight junction proteins, maintaining the integrity of the blood-brain barrier. In the immune system, SIRT1 regulates the activation and differentiation of dendritic cells (DCs) and controls the phenotype and functions of DCs. SIRT1 is a promising target for drug design, with potential applications in treating various diseases, including cancer, neurodegenerative diseases, and inflammatory conditions.

免疫印迹
- WB result of SIRT1 Recombinant Rabbit mAb
  Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 120 kDa
- WB result of SIRT1 Recombinant Rabbit mAb
  Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 110 kDa
  This blot was developed with high sensitivity substrate
- WB result of SIRT1 Recombinant Rabbit mAb
  Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 82 kDa
  Observed MW: 110 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human testis. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human tonsil. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in NIH/3T3 cells. Anti- SIRT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of SIRT1 in tumor tissue.
- Expression of SIRT1 in human tissue.
- Expression of SIRT1 in mouse & rat tissue.