SIRT1 Recombinant Rabbit mAb (S-1886-15)
SirT1,SIR1,SIRT1,SIR2L1,NAD-dependent protein deacetylase sirtuin-1,hSIRT1,NAD-dependent protein deacylase sirtuin-1,Regulatory protein SIR2 homolog 1,SIR2-like protein 1,hSIR2,SIR2-like protein 1 (hSIR2)
货号： S0B1328
- 价格： ￥600
- 规格：
- 数量：
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宿主来源
Rabbit抗原名称
SIRT1分子别名
NAD-dependent protein deacetylase sirtuin-1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); SIR2L1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
Q96EB6克隆号
S-1886-15抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, ICC, WB反应种属 ?
Hu, Ms, Rt阳性样本
HEK-293, HeLa, NIH/3T3, mouse testis, C6, rat testis纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
SIRT1 protein, also known as Silent Information Regulator 1, is a NAD+-dependent deacetylase that plays a crucial role in various cellular processes. It is involved in the regulation of gene expression by histone deacetylation and modulates the acetylation levels of numerous substrates, including p53, PGC-1α, NF-κB, FOXO, NCoR, and p300. SIRT1 is essential for cell cycle control, energy metabolism, response to oxidative stress, cell apoptosis, and aging. Calorie restriction (CR) has been shown to increase SIRT1 expression, which is linked to extended lifespan in mammals. SIRT1 also has anti-inflammatory and anti-aging properties, and its dysregulation is associated with diseases such as mitochondrial complex I deficiency and aging. In the brain, SIRT1 upregulates tight junction proteins, maintaining the integrity of the blood-brain barrier. In the immune system, SIRT1 regulates the activation and differentiation of dendritic cells (DCs) and controls the phenotype and functions of DCs. SIRT1 is a promising target for drug design, with potential applications in treating various diseases, including cancer, neurodegenerative diseases, and inflammatory conditions.
免疫印迹
WB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 120 kDaWB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 110 kDa
This blot was developed with high sensitivity substrateWB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 110 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in NIH/3T3 cells. Anti- SIRT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SIRT1 in tumor tissue.
Expression of SIRT1 in human tissue.
Expression of SIRT1 in mouse & rat tissue.
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