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VDAC1 Recombinant Rabbit mAb (S-1877-18)

Non-selective voltage-gated ion channel VDAC1,Outer mitochondrial membrane protein porin 1,Plasmalemmal porin,Porin 31HL,Porin 31HM,Voltage-dependent anion-selective channel protein 1 (VDAC-1,hVDAC1),VDAC

货号： S0B1321

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
申请试用 斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    VDAC1

  • 分子别名

    Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin, Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1); VDAC

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Endoplasmic reticulum, Mitochondrion, Cell membrane

  • Accession

    P21796

  • 克隆号

    S-1877-18

  • 抗体类型

    Recombinant mAb

  • 应用

    IHC-P, WB

  • 反应种属 ?

    Hu

  • 阳性样本

    HEK-293, HeLa, A431, Jurkat, MCF7

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20°C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu
IHC-P 1:1000 Hu
背景介绍

  • Voltage-dependent anion channel 1 (VDAC1) is a multifunctional protein located in the outer mitochondrial membrane (OMM), playing a crucial role in regulating cellular metabolism and apoptosis. VDAC1 facilitates the transport of metabolites, ions, and small molecules between the mitochondria and the cytosol, including ATP, ADP, and calcium ions, thereby controlling the metabolic cross-talk between these cellular compartments. Additionally, VDAC1 is involved in mitochondria-mediated apoptosis by forming channels that release apoptotic proteins such as cytochrome c into the cytosol. It also interacts with over 150 proteins, including anti-apoptotic proteins like Bcl-2 and hexokinase, which are often overexpressed in cancer cells, making VDAC1 a potential target for cancer therapy.

  • 免疫印迹

    • WB result of VDAC1 Recombinant Rabbit mAb
      Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: unboiled HEK-293 whole cell lysate 20 µg
      Lane 2: unboiled HeLa whole cell lysate 20 µg
      Lane 3: unboiled A431 whole cell lysate 20 µg
      Lane 4: unboiled Jurkat whole cell lysate 20 µg
      Lane 5: unboiled MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 31 kDa
      Observed MW: 32 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human prostate. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of VDAC1 in tumor tissue.

    • Expression of VDAC1 in human tissue.

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