申请试用 VDAC1 Recombinant Rabbit mAb (S-1877-18)
Non-selective voltage-gated ion channel VDAC1,Outer mitochondrial membrane protein porin 1,Plasmalemmal porin,Porin 31HL,Porin 31HM,Voltage-dependent anion-selective channel protein 1 (VDAC-1,hVDAC1),VDAC
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宿主来源
Rabbit抗原名称
VDAC1分子别名
Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin, Porin 31HL; Porin 31HM; Voltage-dependent anion-selective channel protein 1 (VDAC-1; hVDAC1); VDAC免疫原
Recombinant Protein细胞定位
Endoplasmic reticulum, Mitochondrion, Cell membraneAccession
P21796克隆号
S-1877-18抗体类型
Recombinant mAb应用
IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu 反应种属 ?
Hu阳性样本
HEK-293, HeLa, A431, Jurkat, MCF7纯化方式
Protein A浓度
0.5 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
Voltage-dependent anion channel 1 (VDAC1) is a multifunctional protein located in the outer mitochondrial membrane (OMM), playing a crucial role in regulating cellular metabolism and apoptosis. VDAC1 facilitates the transport of metabolites, ions, and small molecules between the mitochondria and the cytosol, including ATP, ADP, and calcium ions, thereby controlling the metabolic cross-talk between these cellular compartments. Additionally, VDAC1 is involved in mitochondria-mediated apoptosis by forming channels that release apoptotic proteins such as cytochrome c into the cytosol. It also interacts with over 150 proteins, including anti-apoptotic proteins like Bcl-2 and hexokinase, which are often overexpressed in cancer cells, making VDAC1 a potential target for cancer therapy.
免疫印迹
WB result of VDAC1 Recombinant Rabbit mAb
Primary antibody: VDAC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled HEK-293 whole cell lysate 20 µg
Lane 2: unboiled HeLa whole cell lysate 20 µg
Lane 3: unboiled A431 whole cell lysate 20 µg
Lane 4: unboiled Jurkat whole cell lysate 20 µg
Lane 5: unboiled MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human prostate. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-VDAC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of VDAC1 in tumor tissue.
Expression of VDAC1 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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