RBM15B Recombinant Rabbit mAb (S-1326-111)

Putative RNA-binding protein 15B,One-twenty two protein 3 (HsOTT3,HuOTT3),RNA-binding motif protein 15B,OTT3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1319

规格

25μl100μl500μl1000μl

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

RBM15B
分子别名

Putative RNA-binding protein 15B; One-twenty two protein 3 (HsOTT3; HuOTT3); RNA-binding motif protein 15B; OTT3
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q8NDT2
克隆号

S-1326-111
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
阳性样本

HeLa, Jurkat, 293T
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
IHC-P 1:250 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
IHC-P	1:250	Hu

背景介绍

RBM15B, also known as RNA binding motif protein 15B, is a member of the SPEN (Split-end) family of proteins and is involved in various cellular processes. It functions as an RNA-binding protein that plays a key role in regulating N6-methyladenosine (m6A) methylation of RNAs, which affects mRNA splicing and processing. RBM15B is also implicated in the regulation of alternative splicing and acts as a cofactor for the nuclear export receptor NXF1, influencing mRNA export from the nucleus. Additionally, it has been associated with diseases such as Tatton-Brown-Rahman Syndrome and Wilms Tumor 1, highlighting its importance in both normal and pathological cellular functions.

免疫印迹
- WB result of RBM15B Recombinant Rabbit mAb
  Primary antibody: RBM15B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: Jurkat whole cell lysate 20 µg
  Lane 3: 293T whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 97 kDa
  Observed MW: 100 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human stomach. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RBM15B antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
- Expression of RBM15B in tumor tissue.
- Expression of RBM15B in human tissue.