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Rabbit抗原名称
MMP7分子别名
Matrilysin; Matrin; Matrix metalloproteinase-7 (MMP-7); Pump-1 protease; Uterine metalloproteinase; MPSL1; PUMP1免疫原
Recombinant Protein细胞定位
Extracellular space, SecretedAccession
P09237克隆号
S-1628-25抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
HT-29, BxPC-3, A549纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:250 Hu
Matrix metalloproteinase-7 (MMP-7) is a small proteolytic enzyme that is secreted as a zinc- and calcium-dependent endopeptidase. It plays a crucial role in the degradation of various extracellular matrix substrates and non-ECM proteins, such as E-cadherin and Fas ligand, which are involved in cell adhesion and apoptosis. MMP-7 is implicated in several physiological processes, including wound healing, bone growth, and remodeling. In cancer, MMP-7 is often overexpressed and contributes to tumor progression by promoting cell proliferation, invasion, metastasis, and angiogenesis. Its expression levels correlate with the clinical characteristics of cancer patients, making it a potential biomarker and therapeutic target for various types of cancer.
免疫印迹
WB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: BxPC-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 25 kDaWB result of MMP7 Recombinant Rabbit mAb
Primary antibody: MMP7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 23, 25 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows negative staining in paraffin-embedded human liver. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-MMP7 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MMP7 in tumor tissue.
Expression of MMP7 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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