HDAC3 Rabbit Polyclonal Antibody

Histone deacetylase 3,HD3,Protein deacetylase HDAC3,Protein deacylase HDAC3,RPD3-2,SMAP45

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1312

规格

25μl100μl500μl1000μl

数量

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产品规格

宿主来源

Rabbit
抗原名称

HDAC3
分子别名

Histone deacetylase 3; HD3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

O15379
抗体类型

Polyclonal antibody
抗体同种型

IgG
应用

ICFCM ,
ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
ICFCM 1:50 Hu, Ms
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, K562, Jurkat, HepG2, MCF7, NIH/3T3, mouse brain, PC-12, C6, rat brain
预测反应种属
(反应种属缩写表)

Hm, Vt
纯化方式

Immunogen Affinity
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu, Ms
ICFCM	1:50	Hu, Ms

背景介绍

Histone deacetylase 3 (HDAC3) is a class I HDAC that plays a critical role in chromatin remodeling and gene transcription regulation. It is unique among HDACs due to its ability to shuttle between the nucleus and cytoplasm, allowing it to exert both enzymatic and non-enzymatic functions. HDAC3 primarily functions as a co-repressor, interacting with nuclear receptor co-repressors (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRT) to deacetylate histones and repress gene expression. This activity is crucial for various physiological processes, including embryonic development, circadian rhythms, and metabolism. Additionally, HDAC3 is implicated in several diseases, such as cancer, neurodegeneration, and cardiovascular diseases, making it a target for therapeutic interventions. Its role in inflammation is also significant, as it regulates pro-inflammatory gene expression in macrophages. HDAC3 inhibitors are being explored for their potential in treating these conditions, with specific inhibitors showing promise in reducing side effects compared to general HDAC inhibitors.

免疫印迹
- WB result of HDAC3 Rabbit pAb
  Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: K562 whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: MCF7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 48 kDa
- WB result of HDAC3 Rabbit pAb
  Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 48 kDa
- WB result of HDAC3 Rabbit pAb
  Primary antibody: HDAC3 Rabbit pAb at 1/1000 dilution
  Lane 1: PC-12 whole cell lysate 20 µg
  Lane 2: C6 whole cell lysate 20 µg
  Lane 3: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 49 kDa
  Observed MW: 48 kDa
流式分析
- Flow cytometric analysis of K562 (Human chronic myelogenous leukemia lymphoblast) labelling HDAC3 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
- Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) labelling HDAC3 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in NIH/3T3 cells. Anti- HDAC3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
- ICC shows positive staining in K562 cells. Anti- HDAC3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).