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CDK8 Recombinant Rabbit mAb (S-1627-70)

Cyclin-dependent kinase 8,Cell division protein kinase 8,Mediator complex subunit CDK8,Mediator of RNA polymerase II transcription subunit CDK8,Protein kinase K35

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1310

规格

25μl100μl500μl1000μl

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CDK8
分子别名

Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
免疫原

Recombinant Protein
细胞定位

Nucleus
Accession

P49336
克隆号

S-1627-70
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

K562, SW480, HCT 116, HT-29, HeLa, NIH/3T3, RAW264.7, mouse testis, rat testis
预测反应种属
(反应种属缩写表)

Sq, Mk, C. aethiops, Rb
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICFCM

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
ICFCM 1:50 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu
ICFCM	1:50	Hu

背景介绍

CDK8 (cyclin-dependent kinase 8) is a serine/threonine kinase that plays a crucial role in regulating DNA transcription and cell cycle progression. It forms a module with Cyclin C (CCNC), Med12, and Med13, which associates with the Mediator complex to modulate gene transcription. CDK8 phosphorylates the C-terminal domain of RNA polymerase II, influencing transcriptional elongation. In cancer, CDK8 acts as a positive regulator of oncogene-induced proliferation by recruiting pTEF-B and BRD4 to oncogenic genes, promoting efficient transcriptional elongation. However, it can also inhibit Mediator-dependent super-enhancer driven transcription in acute myeloid leukemia (AML), suggesting its role may vary depending on the oncogenic context. Additionally, CDK8 is often amplified in colorectal cancer, where it modulates beta-catenin activity, contributing to tumor progression.

免疫印迹
- WB result of CDK8 Recombinant Rabbit mAb
  Primary antibody: CDK8 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: K562 whole cell lysate 20 µg
  Lane 2: SW480 whole cell lysate 20 µg
  Lane 3: HCT 116 whole cell lysate 20 µg
  Lane 4: HT-29 whole cell lysate 20 µg
  Lane 5: HeLa whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 55 kDa
- WB result of CDK8 Recombinant Rabbit mAb
  Primary antibody: CDK8 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Lane 3: mouse testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 55 kDa
- WB result of CDK8 Recombinant Rabbit mAb
  Primary antibody: CDK8 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat testis lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 53 kDa
  Observed MW: 55 kDa
流式分析
- Flow cytometric analysis of HCT116 (Human colorectal carcinoma epithelial cell) labelling CDK8 antibody at 1/50 dilution (1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫细胞化学
- ICC shows positive staining in HCT116 cells. Anti- CDK8 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).