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Rabbit抗原名称
Caspase-7分子别名
CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3 (ICE-LAP3); MCH3; CASP7免疫原
Synthetic Peptide细胞定位
Secreted, Cytoplasm, NucleusAccession
P55210克隆号
S-1539-42抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, Jurkat预测反应种属
(反应种属缩写表)Hm纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,ICC ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu, Ms, Rt ICC 1:500 Hu
Caspase-7 is a cysteine protease that plays a crucial role in the execution phase of apoptosis, the programmed cell death process essential for maintaining tissue homeostasis and development. Along with caspase-3 and caspase-6, it is classified as an executioner caspase, which is activated by initiator caspases such as caspase-8 and caspase-9. Initially thought to be functionally redundant with caspase-3, recent research has highlighted distinct roles for caspase-7. It has unique substrate preferences and is involved in specific cellular processes, including the repair of plasma membrane pores through the activation of acid sphingomyelinase (ASM) under inflammatory conditions. Additionally, caspase-7 is implicated in the regulation of inflammatory responses, particularly through its interaction with inflammasomes. Its distinct functions and involvement in both apoptosis and inflammation make caspase-7 a potential therapeutic target for diseases related to these processes, such as cancer and inflammatory disorders.
免疫印迹
WB result of Caspase-7 Recombinant Rabbit mAb
Primary antibody: Caspase-7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 1 µM staurosporine for 4 hours whole cell lysate 20 µg
Lane 3: untreated HeLa whole cell lysate 20 µg
Lane 4: HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 15, 28, 35 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti- Caspase-7 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in Jurkat cells. Anti- Caspase-7 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Caspase-7 in tumor tissue.
Expression of Caspase-7 in human tissue.
Expression of Caspase-7 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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