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Rabbit抗原名称
ATIC分子别名
Bifunctional purine biosynthesis protein ATIC; AICAR transformylase/inosine monophosphate cyclohydrolase (ATIC); PURH免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P31939抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, HepG2, HT-29, NIH/3T3, C6预测反应种属
(反应种属缩写表)Or, Bv纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu
ATIC protein, also known as 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase, is a bifunctional enzyme that catalyzes the last two steps in the de novo purine biosynthetic pathway. The N-terminal domain of ATIC possesses phosphoribosylaminoimidazolecarboxamide formyltransferase activity, while the C-terminal domain has IMP cyclohydrolase activity . Mutations in the ATIC gene can lead to AICA-ribosiduria, a condition characterized by the accumulation of AICA riboside and its derivatives in the body.
免疫印迹
WB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDaWB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDaWB result of ATIC Rabbit pAb
Primary antibody: ATIC Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 60 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human tonsil. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ATIC antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of ATIC in tumor tissue.
Expression of ATIC in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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