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Apo-D Recombinant Rabbit mAb (S-1869-50)

Apolipoprotein D,ApoD,APOD

价格 600.00 供应商现货 : 3-5个工作日
货号 S0B1306
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产品规格
  • 宿主来源

    Rabbit
  • 抗原名称

    Apo-D
  • 分子别名

    Apolipoprotein D; ApoD; APOD
  • 免疫原

    Synthetic Peptide
  • 细胞定位

    Secreted
  • Accession

    P05090
  • 克隆号

    S-1869-50
  • 抗体类型

    Recombinant mAb
  • 抗体同种型

    IgG
  • 反应种属 ?

    Hu
  • 阳性样本

    SK-MEL-28
  • 纯化方式

    Protein A
  • 浓度

    0.5 mg/ml
  • 标记

    Unconjugated
  • 性状

    Liquid
  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB
  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu
    IHC-P 1:500 Hu
背景介绍
  • Apo-D, or apolipoprotein D, is a glycoprotein with a molecular weight of approximately 33 kDa, primarily produced in the brain and testes rather than the liver, which is typical for most lipoproteins. It is a component of high-density lipoprotein (HDL) and plays a crucial role in lipid metabolism by interacting with lecithin-cholesterol acyltransferase, an enzyme involved in the metabolism of lipoproteins. Apo-D is also implicated in various neurological disorders and is considered a biomarker for conditions such as androgen insensitivity syndrome and certain neurodegenerative diseases. Its function in the nervous system is thought to be related to neuroprotection and repair, possibly due to its antioxidant and anti-inflammatory properties.

  • 免疫印迹

    • WB result of Apo-D Recombinant Rabbit mAb
      Primary antibody: Apo-D Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HepG2 whole cell lysate 20 µg
      Lane 2: SK-MEL-28 whole cell lysate 20 µg
      Negative control: HepG2 whole cell lysate
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 21 kDa
      Observed MW: 23 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human liver. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of Apo-D in tumor tissue.

    • Expression of Apo-D in human tissue.

FAQs

斯达特公司的抗体,可以回收利用几次?

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定

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