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Rabbit抗原名称
Apo-D分子别名
Apolipoprotein D; ApoD; APOD免疫原
Synthetic Peptide细胞定位
SecretedAccession
P05090克隆号
S-1869-50抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
SK-MEL-28纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu
Apo-D, or apolipoprotein D, is a glycoprotein with a molecular weight of approximately 33 kDa, primarily produced in the brain and testes rather than the liver, which is typical for most lipoproteins. It is a component of high-density lipoprotein (HDL) and plays a crucial role in lipid metabolism by interacting with lecithin-cholesterol acyltransferase, an enzyme involved in the metabolism of lipoproteins. Apo-D is also implicated in various neurological disorders and is considered a biomarker for conditions such as androgen insensitivity syndrome and certain neurodegenerative diseases. Its function in the nervous system is thought to be related to neuroprotection and repair, possibly due to its antioxidant and anti-inflammatory properties.
免疫印迹
WB result of Apo-D Recombinant Rabbit mAb
Primary antibody: Apo-D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Negative control: HepG2 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 23 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of Apo-D in tumor tissue.
Expression of Apo-D in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定

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