分享产品介绍 FAQs 评论(0)
宿主来源
Rabbit抗原名称
SNAP25分子别名
Synaptosomal-associated protein 25; Super protein (SUP); SNAP-25; Synaptosomal-associated 25 kDa protein; SNAP免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
P60880克隆号
S-1828-67抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
Human brain, mouse brain, rat brain预测反应种属
(反应种属缩写表)Mq, Gor, Gfs, Zf纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Ms,Rt IHC-P 1:500 Hu, Ms, Rt IF 1:500 Hu, Ms
SNAP25, or synaptosomal-associated protein 25, is a crucial component of the SNARE complex that plays a pivotal role in the molecular regulation of neurotransmitter release. It is primarily associated with the presynaptic membrane and is involved in vesicle docking and membrane fusion, which are essential for the release of neurotransmitters into the synaptic cleft. Recent research has also suggested a potential postsynaptic role for SNAP25, indicating its presence in the postsynaptic density (PSD), postsynaptic lateral membrane, and small vesicles in the postsynaptic cytoplasm, which may be implicated in synaptic plasticity. Furthermore, SNAP25 has been linked to the modulation of the gating characteristics of certain ion channels, such as the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. Its expression and structural abnormalities have been reported in various neurological disorders, emphasizing its importance in synaptic function and neurological health.
免疫印迹
WB result of SNAP25 Recombinant Rabbit mAb
Primary antibody: SNAP25 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse hippocampus lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDaWB result of SNAP25 Recombinant Rabbit mAb
Primary antibody: SNAP25 Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat hippocampus lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 28 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SNAP25 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of SNAP25 in tumor tissue.
Expression of SNAP25 in human tissue.
Expression of SNAP25 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
评论(0)