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宿主来源
Rabbit抗原名称
NRGN分子别名
Neurogranin; Ng; RC3免疫原
Recombinant Protein细胞定位
CytoplasmAccession
Q92686克隆号
S-1624-60抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB, IF反应种属 ?
Hu, Ms, Rt阳性样本
Mouse brain, Rat brain纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| IF | 1:2000 | Hu |
NRGN, or neurogranin, is a brain-specific protein found predominantly in telencephalic neurons. It is known to be a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. NRGN is also under the control of thyroid hormone in specific neuronal subsets, playing a role in the mental states associated with hypothyroidism during development and in adult subjects. The gene is located on chromosome 11q24 and contains four exons and three introns, with exons 1 and 2 encoding the protein. NRGN is considered a direct target for thyroid hormone in the human brain, and its expression is crucial for understanding the impact of thyroid function on cognitive and mental health.
免疫印迹
WB result of NRGN Recombinant Rabbit mAb
Primary antibody: NRGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse lung lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 8 kDa
Observed MW: 15 kDaWB result of NRGN Recombinant Rabbit mAb
Primary antibody: NRGN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat brain lysate 20 µg
Negative control: rat lung lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 8 kDa
Observed MW: 15 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows positive staining in paraffin-embedded human liver. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-NRGN antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded human cerebral cortex. Anti-NRGN antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of NRGN in tumor tissue.
Expression of NRGN in human tissue.
Expression of NRGN in mouse & rat tissue.
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