Phospho-Stat3 (Ser727) Recombinant Rabbit mAb (S-1439-42)

Signal transducer and activator of transcription 3,Acute-phase response factor,APRF

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1290

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-Stat3 (Ser727)
分子别名

Signal transducer and activator of transcription 3; Acute-phase response factor; APRF
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

P40763
克隆号

S-1439-42
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
应用

ChIP ,
WB ,
IP
反应种属 ?

Hu, Ms
预测反应种属
(反应种属缩写表)

Ck, Pg, Bv
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu,Ms
IP	1:50	Hu
ChIP	1:20-1:50

背景介绍

Phospho-Stat3 (Ser727) refers to the phosphorylated form of the Stat3 protein at the serine residue 727. This phosphorylation event is significant for the activation of Stat3, a key transcription factor involved in various cellular processes including cell growth, survival, and immune responses. Phosphorylation at Ser727, although often considered a secondary event following Tyr705 phosphorylation, can occur independently and is associated with increased cell survival activity and nuclear translocation of Stat3. It has been shown to play a role in melanocytes and melanoma cells, where Ser727 phosphorylation can precede Tyr705 phosphorylation in the early stages of melanoma progression. Moreover, the constitutive Ser727 phosphorylation in melanoma cells is partially mediated by the B-Raf–MEK–ERK1/2 pathway, indicating its importance in cell survival and nuclear translocation of Stat3 in melanocytic cells. This modification is also implicated in the regulation of gene expression and cellular metabolism, suggesting its role in tumorigenesis and cancer progression.

免疫印迹
- WB result of Phospho-Stat3 (Ser727) Recombinant Rabbit mAb
  Primary antibody: Phospho-Stat3 (Ser727) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa starve overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 88 kDa
  Observed MW: 90 kDa
免疫沉淀
- Phospho-Stat3 (Ser727) Rabbit mAb at 1/100 dilution (2 µg) immunoprecipitating Phospho-Stat3 (Ser727) in 0.4 mg HeLa starve overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-Stat3 (Ser727) Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa starve overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate 20 µg (Input)
  Lane 2: Phospho-Stat3 (Ser727) Rabbit mAb IP in HeLa starve overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa starve overnight, then treated with 200 nM TPA for 30 minutes whole cell lysate
  Predicted MW: 88 kDa
  Observed MW: 90 kDa
染色质免疫沉淀
- Chromatin immunoprecipitation (ChIP) was performed on HeLa+IFN-α (50 ng/ml, 30 min) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Phospho-Stat3 (Ser727) Recombinant Rabbit mAb (S-1439-42)and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of c-FOS, IRF1 and SAT-α in STAT3 (pS727) Recombinant Rabbit mAb (S-1439-42)-immunoprecipitated sample.
斑点杂交
- Dot blot result of Phospho-Stat3 (Ser727) Recombinant Rabbit mAb
  Lane 1: Stat3 (Ser727) phospho peptide
  Lane 2: Stat3 unmodified peptide
  Primary antibody: Phospho-Stat3 (Ser727) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution