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Rabbit抗原名称
Rab11分子别名
Ras-related protein Rab-11A; Rab-11; YL8; RAB11A免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
P62491克隆号
S-1601-68抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P ? ,ICC ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:250 Hu ICC 1:500 Hu 反应种属 ?
Hu, Ms, Rt阳性样本
A431, HeLa, Jurkat, MCF7, A549, Neuro-2a, C6纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
Rab11 is a member of the Rab family of small GTPases that plays a pivotal role in membrane trafficking, particularly in the recycling of membrane components from endosomes back to the plasma membrane. It is predominantly found in recycling endosomes and is crucial for the transport of vesicles from these endosomes to the cell surface, facilitating the reuse of receptors and membrane proteins. Its function has been well-defined in the recycling pathway, but its role in early endosomes is less understood. Recent studies have also implicated Rab11 in the balance of the endosome-lysosome pathway, suggesting its broader impact on cellular homeostasis. Overall, Rab11 is a key regulator in the maintenance of cellular membrane dynamics and the control of intracellular trafficking routes.
免疫印迹
WB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDaWB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDaWB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in A549 cells. Anti-Rab11 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of Rab11 in tumor tissue.
Expression of Rab11 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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