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Rabbit抗原名称
Aquaporin 5/AQP5分子别名
AQP-5免疫原
Synthetic Peptide细胞定位
Cell membrane, Apical cell membraneAccession
P55064克隆号
S-1622-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
KATO Ⅲ, mouse lung, rat lung纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IF ?
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu IF 1:500 Ms
Aquaporin 5 (AQP5) is a water channel protein that plays a significant role in the generation of saliva, tears, and pulmonary secretions. It is a member of the major intrinsic protein family, characterized by its ability to selectively transport water across cell membranes. AQP5 is predominantly expressed in the salivary and lacrimal glands, as well as in the respiratory tract, and is crucial for maintaining water homeostasis within these secretions. The protein is also implicated in the pathogenesis of certain diseases, such as Sjögren's syndrome, where its abnormal subcellular distribution in salivary gland cells may contribute to the disease's development.
免疫印迹
WB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled KATO Ⅲ whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDaWB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse kidney lysate 20 µg
Lane 2: unboiled mouse lung lysate 20 µg
Negative control: unboiled mouse kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDaWB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat kidney lysate 20 µg
Lane 2: unboiled rat lung lysate 20 µg
Negative control: unboiled rat kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
IF shows positive staining in paraffin-embedded mouse lung. Anti- Aquaporin 5/AQP5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
组织表达图谱
Expression of Aquaporin 5/AQP5 in tumor tissue.
Expression of Aquaporin 5/AQP5 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
引用文献(1)
- Effect of Maternal Administration with All-Trans Retinoic Acid on Lungs of Neonatal Pigs
Xiao X, Zhou H, He D, Wei P, Zhu Y, Sun W, Hao S, Wu H, Lu W, He Y.
Vet Sci. 2025 Nov 28 .
影响因子： 2.3
货号：S0B1287产品名称：Aquaporin 5/AQP5 Recombinant Rabbit mAb (S-1622-5)
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