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Rat抗原名称
Mouse CD14分子别名
Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein细胞定位
Cell membraneAccession
P10810克隆号
S-R609抗体类型
Rat mAb抗体同种型
IgG2a,k应用
FCM反应种属 ?
Ms阳性样本
RAW 264.7纯化方式
Protein G浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:200 | Ms |
CD14, also known as the LPS receptor, is a surface antigen belonging to the family of cell surface glycoproteins. It exists in two forms: membrane-bound CD14 (mCD14), which is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) tail, and soluble CD14 (sCD14), found in the plasma. CD14 is primarily expressed on monocytes, macrophages, and dendritic cells, and plays a critical role in the immune system by recognizing and binding to lipopolysaccharide (LPS) from Gram-negative bacteria, leading to cellular activation and the initiation of inflammatory responses.
流式分析
Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) labelling Mouse CD14 antibody at 1/200 dilution (1 μg) / (Red) compared with a Rat IgG2a, κ isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody.
Negative control: Neuro-2a
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