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Rabbit抗原名称
Phospho-NF-κB p105 (Ser932)分子别名
Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P19838克隆号
S-1146-117抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Ms, Rt预测反应种属
(反应种属缩写表)Dg纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt
Phospho-NF-κB p105 (Ser932) refers to the phosphorylated form of the NF-κB p105 protein at the serine residue 932. This post-translational modification is significant as it triggers a series of events that lead to the activation of the NF-κB signaling pathway, which is crucial in regulating immune and inflammatory responses. When p105 is phosphorylated at Ser932 by the IKK complex, it recruits the SCFβTrCP E3 ligase, leading to the ubiquitination and subsequent proteasomal degradation of p105. This degradation releases the active form of TPL-2, which in turn activates the MEK/ERK signaling pathway. Therefore, phosphorylation at Ser932 is a key regulatory step in controlling NF-κB activation and MAP kinase signaling, which coordinate the functions of lymphocytes and macrophages in the immune response.
免疫印迹
WB result of Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Lane 3: NIH/3T3 treated with 20 ng/ml TNF-α for 10 minutes and 100 nM Calyculin A for 10 minutes, then treated with phosphatase whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 110 kDaWB result of Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-κB p105 (Ser932) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 110 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高
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