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Rabbit抗原名称
SHMT2/SHMT分子别名
Serine hydroxymethyltransferase, mitochondrial; Glycine hydroxymethyltransferase; Serine methylase免疫原
Synthetic Peptide细胞定位
Cytoplasm, Nucleus, MitochondrionAccession
P34897克隆号
S-1581-5抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
PC-3, MCF7, HeLa, HepG2, OVCAR-3, mouse liver, rat liver纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:5000 Hu, Ms, Rt IP 1:50 IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu
SHMT2 protein, or serine hydroxymethyltransferase 2, is a mitochondrial enzyme that plays a pivotal role in one-carbon metabolism by catalyzing the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction is crucial for the synthesis of glycine and the provision of one-carbon units necessary for DNA synthesis, particularly through the production of thymidine and pyrimidine nucleotides. SHMT2 is expressed ubiquitously, with particularly high levels in the liver, and its activity is suggested to be the primary source of intracellular glycine. It is also associated with maintaining normal cellular methylation patterns, DNA stability, and genetic mutations. Abnormal expression of SHMT2 has been linked to various cancers, including colorectal, renal cell, head and neck, hepatocellular carcinoma, non-small cell lung cancer, breast cancer, and prostate cancer, where it contributes to cancer cell proliferation and survival by supporting metabolic reprogramming. Therefore, SHMT2 is not only a key player in cellular metabolism but also a potential therapeutic target in cancer treatment.
免疫印迹
WB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: OVCAR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDaWB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDaWB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDa
免疫沉淀
SHMT2/SHMT Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SHMT2/SHMT in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SHMT2/SHMT Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: SHMT2/SHMT Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 56 kDa
Observed MW: 50 kDaSHMT2/SHMT Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SHMT2/SHMT in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SHMT2/SHMT Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: SHMT2/SHMT Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 56 kDa
Observed MW: 50 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti-SHMT2/SHMT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of SHMT2/SHMT in tumor tissue.
Expression of SHMT2/SHMT in human tissue.
Expression of SHMT2/SHMT in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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