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Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb (S-1124-133)

Transcription factor p65,Nuclear factor NF-kappa-B p65 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3,RELA,NFKB3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1280

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Phospho-NF-κB p65 (Ser468)
分子别名

Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q04206
克隆号

S-1124-133
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu,Ms,Rt
IP 1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu,Ms,Rt
IP	1:50	Hu

背景介绍

Phospho-NF-κB p65 (Ser468) refers to the phosphorylation of the p65 subunit of NF-κB at serine 468. This specific phosphorylation event plays a distinct role in the regulation of NF-κB signaling. Unlike many other phosphorylation sites on p65 that generally enhance its transcriptional activity, phosphorylation at Ser468 has been shown to inhibit p65's transactivation potential and can be involved in p65 ubiquitination and degradation, thereby reducing its transcriptional activity. This modification is part of the complex post-translational regulation of NF-κB, which is a critical mediator of cellular responses to various stimuli, including inflammation, immune responses, and cellular stress. The phosphorylation status of p65 at Ser468 can impact the cell's inflammatory response and may have implications for diseases where NF-κB signaling is dysregulated.

免疫印迹
- WB result of Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 60 kDa
  Observed MW: 70 kDa
- WB result of Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 starve for 3hr then treated with Calyculin A (100nM/ml, 30min) whole cell lysate 20 µg
  Negative control: Untreated NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
  Observed MW: 70kDa
- WB result of Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: Untreated C6 whole cell lysate 20 µg
  Lane 2: C6 starve overnight then treated with TNF-α (20ng/ml, 30min) and Calyculin A (100nM/ml, 30min) whole cell lysate 20 µg
  Negative control: Untreated C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa
  Observed MW: 70kDa
免疫沉淀
- Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating phospho-NF-κB p65 (Ser468) in 0.4 mg HeLa + TNF-alpha (20 ng/ml) and Calyculin A (50 nM) for 5 mins whole cell lysate.
  Western blot was performed on the immunoprecipitate using Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa + TNF-alpha (20 ng/ml) and Calyculin A (50 nM) for 5 mins whole cell lysate 20 µg (Input)
  Lane 2: Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb IP in HeLa + TNF-alpha (20 ng/ml) and Calyculin A (50 nM) for 5 mins whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa + TNF-alpha (20 ng/ml) and Calyculin A (50 nM) for 5 mins whole cell lysate
  Predicted MW: 60 kDa
  Observed MW: 70 kDa
  Exposure time: 超敏90 s
斑点杂交
- Dot blot result of Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb
  Lane 1: NF-κB p65 (Ser468) phospho peptide
  Lane 2: NF-κB p65 unmodified peptide
  Primary antibody: Phospho-NF-κB p65 (Ser468) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution