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宿主来源
Rabbit抗原名称
Whitlow/218 Linker免疫原
Synthetic Peptide克隆号
S-712-10抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM稀释度
应用 稀释度 推荐种属 FCM 1:20000 Species independent
Whitlow 218 Linker is a peptide linker used in the construction of single-chain variable fragments (scFv), designed to reduce aggregation and enhance proteolytic stability. It plays a crucial role in chimeric antigen receptor (CAR) technology by improving the recognition capability and stability of scFv against target antigens, thereby serving as a key component in immunotherapy and biomedical research.
流式分析
Flow cytometric analysis of ScFv based CAR containing a Whitlow/218 linker transfected 293T (Human embryonic kidney epithelial cell (Right panel) or untrasfected 293T (Left panel) labelling Whitlow/218 Linker antibody at 1/5000 (0.01 μg) dilution. Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
示意图
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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