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TIM-1 Recombinant Rabbit mAb (S-1555-147)

Hepatitis A virus cellular receptor 1,HAVcr-1,Kidney injury molecule 1 (KIM-1),T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1),T-cell immunoglobulin mucin receptor 1 (TIM,TIM-1),T-cell membrane protein 1,CD365,HAVCR1,KIM1,TIM1,TIMD1

货号： S0B1274

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    TIM-1

  • 分子别名

    Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1 (KIM-1); T-cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1); T-cell immunoglobulin mucin receptor 1 (TIM; TIM-1); T-cell membrane protein 1; CD365; HAVCR1; KIM1; TIM1; TIMD1

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    Q96D42

  • 克隆号

    S-1555-147

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu

  • 阳性样本

    HEK-293, Caco-2, HeLa

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu
IHC-P 1:250 Hu
ICC 1:500 Hu
背景介绍

  • TIM-1 protein, also known as T-cell immunoglobulin and mucin domain protein-1 or kidney injury molecule-1 (KIM-1), is a type I membrane protein that plays a significant role in immune regulation. It is structurally a member of the immunoglobulin superfamily (IgSF), containing an IgV-like domain, a mucin domain, a transmembrane domain, and an intracellular cytoplasmic tail with a tyrosine phosphorylation motif. TIM-1 functions as a co-stimulatory molecule for T cell activation and is preferentially expressed on activated Th2 cells, influencing cytokine production and allergic diseases. It has also been identified as a cellular receptor for hepatitis A virus and is implicated in the clearance of apoptotic cells, suggesting its involvement in immune tolerance and the prevention of autoimmune and inflammatory diseases. Furthermore, TIM-1 is upregulated in acute kidney injury and has potential as a cancer marker, highlighting its importance in both physiological and pathological conditions.

  • 免疫印迹

    • WB result of TIM-1 Recombinant Rabbit mAb
      Primary antibody: TIM-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HEK-293 whole cell lysate 20 µg
      Lane 2: Caco-2 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 39 kDa
      Observed MW: 55 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human papillary renal cell carcinoma. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human papillary renal cell paracancerous. Anti- TIM-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- TIM-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in HEK293 cells. Anti- TIM-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of TIM-1 in tumor tissue.

    • Expression of TIM-1 in human tissue.

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