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Talin-1 Recombinant Rabbit mAb (S-1483-25)

TLN1,KIAA1027,TLN

货号： S0B1273

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
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产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Talin-1

  • 分子别名

    TLN1; KIAA1027; TLN

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoskeleton, Cytoplasm

  • Accession

    Q9Y490

  • 克隆号

    S-1483-25

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, HepG2, HUVEC, K562, THP-1, Ramos, NIH/3T3, C6

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu, Ms
背景介绍

  • Talin-1 protein, also known as TLN1, is a crucial cytoskeletal protein that plays a significant role in cell adhesion, migration, and mechanotransduction. It is the primary activator of integrins, linking the cytoplasmic domain of integrins to the actin cytoskeleton to form focal adhesions, which are essential for cell-extracellular matrix interactions. Talin-1 is composed of an N-terminal FERM domain and a C-terminal rod domain, which includes multiple helical bundles and a dimerization domain. This protein is mechanosensitive, allowing it to unfold in response to mechanical stress and reveal various binding sites, thus converting mechanical signals into chemical signals through multiple signaling pathways. Talin-1 is also implicated in the regulation of cell growth, proliferation, and differentiation by modulating integrin activity and participating in both 'inside-out' and 'outside-in' signaling. Its expression levels vary across different tumor types and have been associated with cancer progression and tumorigenesis, highlighting its potential as a therapeutic target in oncology.

  • 免疫印迹

    • WB result of Talin-1 Recombinant Rabbit mAb
      Primary antibody: Talin-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: HepG2 whole cell lysate 20 µg
      Lane 3: HUVEC whole cell lysate 20 µg
      Lane 4: K562 whole cell lysate 20 µg
      Lane 5: THP-1 whole cell lysate 20 µg
      Lane 6: Ramos whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 270 kDa
      Observed MW: 250~280 kDa

    • WB result of Talin-1 Recombinant Rabbit mAb
      Primary antibody: Talin-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 270 kDa
      Observed MW: 250~280 kDa

    • WB result of Talin-1 Recombinant Rabbit mAb
      Primary antibody: Talin-1 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 270 kDa
      Observed MW: 250~280 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human stomach. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat colon. Anti- Talin-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti- Talin-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

    • ICC shows positive staining in NIH/3T3 cells. Anti- Talin-1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of Talin-1 in tumor tissue.

    • Expression of Talin-1 in human tissue.

    • Expression of Talin-1 in mouse & rat tissue.

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