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MCP1 Recombinant Rabbit mAb (S-1737-3)

C-C motif chemokine 2,HC11,Monocyte chemoattractant protein 1,Monocyte chemotactic and activating factor (MCAF),Monocyte chemotactic protein 1 (MCP-1),Monocyte secretory protein JE,Small-inducible cytokine A2,CCL2,SCYA2

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1269

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

MCP1
分子别名

C-C motif chemokine 2; HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor (MCAF); Monocyte chemotactic protein 1 (MCP-1); Monocyte secretory protein JE; Small-inducible cytokine A2; CCL2; SCYA2
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P13500
克隆号

S-1737-3
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu
预测反应种属
(反应种属缩写表)

Or
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu
ICC	1:500	Hu

背景介绍

MCP-1, also known as CCL2, is a chemokine that plays a central role in inflammation and immune responses. It is primarily produced by various cell types, including monocytes, macrophages, endothelial cells, fibroblasts, and epithelial cells. MCP-1 is crucial for the recruitment, activation, and migration of leukocytes, particularly monocytes and macrophages, to the site of inflammation. It is involved in the pathogenesis of numerous diseases, including COVID-19, cancers, neuroinflammatory diseases, rheumatoid arthritis, and cardiovascular diseases, by promoting the migration and infiltration of inflammatory cells. Additionally, MCP-1 contributes to insulin resistance, diabetes, and its complications, such as diabetic nephropathy and retinopathy, by increasing the level of circulating MCP-1 in type 1 and type 2 diabetes. Furthermore, it plays a role in cancer by recruiting macrophages to the tumor microenvironment, promoting angiogenesis, and inducing cell invasion and metastasis. Overall, MCP-1 is a key chemoattractant with functions that extend beyond immunity, influencing a wide range of pathological processes.

免疫印迹
- WB result of MCP1 Recombinant Rabbit mAb
  Primary antibody: MCP1 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 20 ng/ml TNF-alpha for 6 hours and 1 µg/ml Brefeldin A for 3 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 11 kDa
  Observed MW: 11~13 kDa
免疫细胞化学
- ICC analysis of HeLa cells treated with TNF-alpha(20 ng/ml, 6 hr)add Brefeldin A (1 µg/m, 3 hr) (top panel) and untreated HeLa cells (below panel). Anti- MCP1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).