Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-1664-7)

H3K14ac

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1268

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Histone H3 (acetyl K14)
分子别名

H3K14ac
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

P68431
克隆号

S-1664-7
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

乙酰化
反应种属 ?

Hu
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1:1000 Hu
ChIP 1:20-1:50 Hu

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1:1000	Hu
ChIP	1:20-1:50	Hu

背景介绍

Histone H3 (acetyl K14), or H3K14ac, is a specific post-translational modification of the histone H3 protein at lysine 14, which plays a crucial role in chromatin structure and gene regulation. This acetylation event is particularly important for the silencing of ribosomal DNA (rDNA) and the aging process. H3K14 acetylation is also implicated in the regulation of replicative aging through its influence on replication-dependent nucleosome assembly. Moreover, H3K14ac has been shown to facilitate DNA repair within positioned nucleosomes by stabilizing the binding of the chromatin remodeler RSC (Remodels the Structure of Chromatin), thereby playing a significant role in the response to UV damage. This modification is generally associated with gene activation and is regulated by the balance of histone acetyltransferases (HATs) and histone deacetylases (HDACs), which add or remove acetyl groups from lysine residues on histone tails, respectively.

免疫印迹
- WB result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
  Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 15 kDa
  Observed MW: 17 kDa
  This blot was developed with high sensitivity substrate
斑点杂交
- Dot blot result of Histone H3 (acetyl K14) Recombinant Rabbit mAb
  Lane 1: Histone H3K14 acetyl peptide
  Lane 2: Histone H3 unmodified peptide
  Primary antibody: Histone H3 (acetyl K14) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HeLa + Sodium butyrate (30mM,4hr) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used Histone H3 (acetyl K14) Recombinant Rabbit mAb (S-1664-7) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  
  qPCR (%input: immunoprecipitated DNA/input DNA)
  showed the enrichment of RPL30, GAPDH, MYOD1,
  AFM, SAT-α and SAT-2 in Histone H3 (acetyl K14) Recombinant Rabbit
  mAb (S-1664-7)-immunoprecipitated sample.