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Rabbit抗原名称
IMPDH2分子别名
Inosine-5'-monophosphate dehydrogenase 2; IMP dehydrogenase 2; IMPD 2; IMPDH 2; Inosine-5'-monophosphate dehydrogenase type II (IMP dehydrogenase II; IMPDH-II); IMPD2免疫原
Synthetic Peptide细胞定位
Nucleus, CytoplasmAccession
P12268抗体类型
Polyclonal antibody抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
K562, HeLa, HCT 116, NIH/3T3, PC-12纯化方式
Immunogen Affinity浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
IHC-P ?
ICC ?
WB
IP
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms, Rt IP 1:50 Hu IHC-P 1:1000 Hu, Ms, Rt ICC 1:500 Hu, Ms ICFCM 1:500 Hu, Ms
IMPDH2, or inosine monophosphate dehydrogenase 2, is a critical enzyme that catalyzes the first committed step in the de novo synthesis of guanine nucleotides, which are essential for DNA and RNA synthesis. It plays a significant role in cellular proliferation and the immune response. The protein is known to be upregulated in proliferating tissues and is associated with the development of some tumors, suggesting a role in malignant transformation. IMPDH2 has also been shown to interact with PARP1 on chromatin, modulating the DNA damage response by controlling PARP1 activity and nuclear NAD+ levels, which is crucial for cell survival and the balance of DNA damage repair. Additionally, IMPDH2 can reversibly polymerize in response to metabolic demands, and this self-assembly is recognized as a mechanism for regulating its activity, particularly by making the enzyme less sensitive to feedback inhibition under conditions that require an expansion of guanine nucleotide pools.
免疫印迹
WB result of IMPDH2 Rabbit pAb
Primary antibody: IMPDH2 Rabbit pAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HCT 116 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 55 kDaWB result of IMPDH2 Rabbit pAb
Primary antibody: IMPDH2 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDaWB result of IMPDH2 Rabbit pAb
Primary antibody: IMPDH2 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDa
流式分析
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labelling IMPDH2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) labelling IMPDH2 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫沉淀
IMPDH2 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating IMPDH2 in 0.4 mg K-562 whole cell lysate.
Western blot was performed on the immunoprecipitate using IMPDH2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K-562 whole cell lysate 20 µg (Input)
Lane 2: IMPDH2 Rabbit mAb IP in K-562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K-562 whole cell lysate
Predicted MW: 56 kDa
Observed MW: 55 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-IMPDH2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-IMPDH2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-IMPDH2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-IMPDH2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in HeLa cells. Anti- IMPDH2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-IMPDH2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of IMPDH2 in human tissue.
Expression of IMPDH2 in tumor tissue.
Expression of IMPDH2 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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