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宿主来源
Mouse抗原名称
IL-6分子别名
Interleukin-6; B-cell stimulatory factor 2 (BSF-2); CTL differentiation factor (CDF); Hybridoma growth factor; Interferon beta-2 (IFN-beta-2); IL6; IFNB2免疫原
Recombinant Protein细胞定位
SecretedAccession
P05231克隆号
S-1038-139抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1:2000 Hu
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
流式分析
Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized THP-1 (Human monocytic leukemia monocyte), treated overnight with 500ng/ml LPS (Red) or untreated (Green), labeling IL-6 at 1/2000 dilution (0.1 μg) compared with a Mouse monoclonal IgG isotype control (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Mouse IgG Alexa Fluor® 488 was used as the secondary antibody.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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