Prolactin/PRL Recombinant Rabbit mAb (S-235-215)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1260

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Prolactin/PRL
免疫原

Recombinant Protein
细胞定位

Secreted
Accession

P01236
克隆号

S-235-215
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

Human pituitary, mouse pituitary, rat pituitary
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ? ,
WB ,
IF ?
稀释度

应用稀释度推荐种属

WB 1:1000 Ms, Rt
IHC-P 1:2000 Hu, Ms, Rt
IF 1:2000 Hu

应用	稀释度	推荐种属
WB	1:1000	Ms, Rt
IHC-P	1:2000	Hu, Ms, Rt
IF	1:2000	Hu

背景介绍

Prolactin is a hormone primarily synthesized and released by the anterior pituitary gland, known for its crucial roles in lactation, mammary gland development, and milk production, with additional functions in immune regulation, metabolism, and reproduction across various systems in the body.

免疫印迹
- WB result of Prolactin/PRL Recombinant Rabbit mAb
  Primary antibody: Prolactin/PRL Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Lane 2: mouse pituitary lysate 20 µg
  Negative control: mouse spleen lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26 kDa
  Observed MW: 23 kDa
- WB result of PRL Recombinant Rabbit mAb
  Primary antibody: Prolactin/PRL Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat spleen lysate 20 µg
  Lane 2: rat pituitary lysate 20 µg
  Negative control: mouse spleen lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 26 kDa
  Observed MW: 23 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human pituitary. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse pituitary. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat pituitary. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- Negative control: IHC shows negative staining in paraffin-embedded rat cerebral cortex. Anti-Prolactin/PRL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫荧光
- IF shows positive staining in paraffin-embedded human pituitary. Anti-Prolactin PRL antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.