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宿主来源
Rabbit抗原名称
NPHS2分子别名
Podocin免疫原
Synthetic Peptide细胞定位
Cell membraneAccession
Q9NP85克隆号
S-1824-36抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu阳性样本
Human kidney纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
稀释度
应用 稀释度 推荐种属 IHC-P 1:500 Hu
NPHS2 protein, also known as podocin, is a key component of the slit diaphragm in the glomerular filtration barrier and plays a crucial role in regulating glomerular permeability; mutations in this protein can lead to steroid-resistant nephrotic syndrome.
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-NPHS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-NPHS2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of NPHS2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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