热搜词：

CN

  • CN
  • EN

注册

产品中心 抗体 其他一抗

所有图片（1/12）

nmt55/p54nrb Recombinant Rabbit mAb (S-1745-48)

Non-POU domain-containing octamer-binding protein,NonO protein,54 kDa nuclear RNA- and DNA-binding protein (p54(nrb),p54nrb),55 kDa nuclear protein (NMT55),DNA-binding p52/p100 complex,52 kDa subunit,NONO,NRB54

货号： S0B1258

Datasheet COA
  • 价格： ￥600
  • 规格：
  • 数量：
申请试用 斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    nmt55/p54nrb

  • 分子别名

    Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein (p54(nrb); p54nrb); 55 kDa nuclear protein (NMT55); DNA-binding p52/p100 complex, 52 kDa subunit; NONO; NRB54

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Nucleus

  • Accession

    Q15233

  • 克隆号

    S-1745-48

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 应用

    IHC-P, ICC, WB

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, 293T, Jurkat, MCF7, Molt-4, PC-3

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

稀释度
应用 稀释度 推荐种属
WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu
背景介绍

  • p54nrb, also known as NONO, is a multifunctional nuclear protein that plays a role in various nuclear processes, including transcriptional control, RNA splicing and editing, and DNA unwinding and pairing. It is involved in the nuclear retention of defective RNAs and RNA transport, and its disruption can lead to expressional changes of tumorigenic genes and decreased stress tolerance, resulting in increased cell death susceptibility in human tumor cells. Additionally, p54nrb is multiphosphorylated during mitosis and interacts with the mitotic regulator Pin1, suggesting its involvement in cell cycle regulation.

  • 免疫印迹

    • WB result of nmt55/p54nrb Recombinant Rabbit mAb
      Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: 293T whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: MCF7 whole cell lysate 20 µg
      Lane 5: Molt-4 whole cell lysate 20 µg
      Lane 6: PC-3 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 60 kDa

    • WB result of nmt55/p54nrb Recombinant Rabbit mAb
      Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: RAW264.7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 60 kDa

    • WB result of nmt55/p54nrb Recombinant Rabbit mAb
      Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: C6 whole cell lysate 20 µg
      Lane 2: PC-12 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 54 kDa
      Observed MW: 60 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human breast cancer. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti- nmt55/p54nrb antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).

  • 组织表达图谱

    • Expression of nmt55/p54nrb in tumor tissue.

    • Expression of nmt55/p54nrb in human tissue.

    • Expression of nmt55/p54nrb in mouse & rat tissue.

    评论(0)