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nmt55/p54nrb Recombinant Rabbit mAb (S-1745-48)

Non-POU domain-containing octamer-binding protein,NonO protein,54 kDa nuclear RNA- and DNA-binding protein (p54(nrb),p54nrb),55 kDa nuclear protein (NMT55),DNA-binding p52/p100 complex,52 kDa subunit,NONO,NRB54

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1258

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

nmt55/p54nrb
分子别名

Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein (p54(nrb); p54nrb); 55 kDa nuclear protein (NMT55); DNA-binding p52/p100 complex, 52 kDa subunit; NONO; NRB54
免疫原

Synthetic Peptide
细胞定位

Nucleus
Accession

Q15233
克隆号

S-1745-48
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, 293T, Jurkat, MCF7, Molt-4, PC-3
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

IHC-P ?

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IHC-P 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IHC-P	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

p54nrb, also known as NONO, is a multifunctional nuclear protein that plays a role in various nuclear processes, including transcriptional control, RNA splicing and editing, and DNA unwinding and pairing. It is involved in the nuclear retention of defective RNAs and RNA transport, and its disruption can lead to expressional changes of tumorigenic genes and decreased stress tolerance, resulting in increased cell death susceptibility in human tumor cells. Additionally, p54nrb is multiphosphorylated during mitosis and interacts with the mitotic regulator Pin1, suggesting its involvement in cell cycle regulation.

免疫印迹
- WB result of nmt55/p54nrb Recombinant Rabbit mAb
  Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: 293T whole cell lysate 20 µg
  Lane 3: Jurkat whole cell lysate 20 µg
  Lane 4: MCF7 whole cell lysate 20 µg
  Lane 5: Molt-4 whole cell lysate 20 µg
  Lane 6: PC-3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 60 kDa
- WB result of nmt55/p54nrb Recombinant Rabbit mAb
  Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Lane 2: RAW264.7 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 60 kDa
- WB result of nmt55/p54nrb Recombinant Rabbit mAb
  Primary antibody: nmt55/p54nrb Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Lane 2: PC-12 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 54 kDa
  Observed MW: 60 kDa
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human breast cancer. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-nmt55/p54nrb antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti- nmt55/p54nrb antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
- Expression of nmt55/p54nrb in tumor tissue.
- Expression of nmt55/p54nrb in human tissue.
- Expression of nmt55/p54nrb in mouse & rat tissue.