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Rabbit抗原名称
UCP1分子别名
Mitochondrial brown fat uncoupling protein 1; Solute carrier family 25 member 7; Thermogenin; Slc25a7; Ucp免疫原
Synthetic PeptideAccession
P12242克隆号
S-1571-45抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms, Rt阳性样本
mouse brown adipose, rat brown adipose纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms, Rt
UCP1 protein, or uncoupling protein 1, is a mitochondrial carrier found predominantly in brown adipose tissue (BAT) that plays a crucial role in non-shivering thermogenesis. It facilitates the return of protons to the mitochondrial matrix, dissipating the protonmotive force and thereby generating heat instead of ATP, which is vital for maintaining body temperature and energy balance. UCP1 is activated by fatty acids and is inhibited by purine nucleotides, and its unique function is essential for adaptive thermogenesis in response to cold and metabolic challenges.
免疫印迹
WB result of UCP1 Recombinant Rabbit mAb
Primary antibody: UCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brown adipose lysate 20 µg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDaWB result of UCP1 Recombinant Rabbit mAb
Primary antibody: UCP1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brown adipose lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33 kDa
Observed MW: 30 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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