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宿主来源
Rabbit抗原名称
Histone H3 (acetyl K9)分子别名
H3K9ac免疫原
Synthetic Peptide细胞定位
NucleusAccession
P68431克隆号
S-1704-76抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Ar, Bv, C.el, Dr, Fu, Hm, Or, Pl, Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ChIP
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt IHC-P 1:500 Hu, Ms, Rt ChIP 1:20~1:50 Hu
Histone H3 (acetyl K9), or H3K9ac, is a specific modification of the histone protein H3, where the lysine at position 9 is acetylated. This epigenetic mark is associated with active gene transcription and an open chromatin state, which facilitates the access of the transcription machinery to DNA. H3K9ac plays a role in recruiting proteins important for transcription elongation, such as the super elongation complex (SEC), thereby promoting the transition from transcription initiation to elongation. It is also implicated in the regulation of gene expression, with studies suggesting that it can influence the binding of other proteins to chromatin and affect the overall chromatin structure. Moreover, H3K9ac is involved in the balance between acetylation and methylation of histone H3 on lysine 9, which is crucial for the regulation of gene expression and the establishment of chromatin domains. This modification is part of the broader histone code, which contributes to the regulation of chromatin states and gene expression in response to various cellular signals and developmental cues.
免疫印迹
WB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 1 μM TSA for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 400 nM TSA for 18 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDaWB result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 17 kDa
斑点杂交
Dot blot result of Histone H3 (acetyl K9) Recombinant Rabbit mAb
Lane 1: Histone H3K9ac modified peptide
Lane 2: Histone H3K9 unmodified peptide
Primary antibody: Histone H3 (acetyl K9) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Histone H3 (acetyl K9) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
ChIP
Chromatin immunoprecipitation (ChIP) was performed on HeLa+TSA (500ng/ml,4h) (+) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication.
Parallel reactions used Histone H3 (acetyl K9) Recombinant Rabbit mAb (S-1704-76) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
qPCR (%input: immunoprecipitated DNA/input DNA)
showed the enrichment of RPL30, GAPDH, MYOD1,
AFM, SAT-α and SAT-2 in Histone H3 (acetyl K9)
Recombinant Rabbit mAb (S-1704-76)-immunoprecipitated
sample.
组织表达图谱
Expression of Histone H3 (acetyl K9) in tumor tissue.
Expression of Histone H3 (acetyl K9) in human tissue.
Expression of Histone H3 (acetyl K9) in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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