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Rabbit抗原名称
EHD2分子别名
EH domain-containing protein 2; PAST homolog 2; PAST2免疫原
Synthetic Peptide细胞定位
Cytoplasm, Cell membraneAccession
Q9NZN4克隆号
S-1580-60抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
HeLa, A549, HCT 116纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:1000 Hu, Ms, Rt
EHD2, a member of the EH domain-containing protein family, is primarily located in the plasma membrane but can also be found in the cytoplasm and endosomes. It functions as a nuclear-cytoplasmic shuttle protein and acts as a corepressor of transcription within the nucleus to inhibit gene transcription. EHD2 plays a key role in endocytic transport, being involved in the formation and maintenance of endosomal tubules and vesicles, which are essential for intracellular transport of proteins and other substances. The N-terminus of EHD2 is attached to the cell membrane, while its C-terminus binds to actin-binding proteins, linking the actin cytoskeleton to membrane curvature and promoting cell endocytosis. Additionally, EHD2 is associated with membrane protein trafficking, receptor signaling, glucose metabolism, and lipid metabolism, highlighting its multifaceted role in cellular processes and its potential implications in diseases such as cancer and metabolic disorders.
免疫印迹
WB result of EHD2 Recombinant Rabbit mAb
Primary antibody: EHD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: SW620 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Negative control: SW620 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-EHD2 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of EHD2 in tumor tissue.
Expression of EHD2 in human tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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