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别名
Frizzled5,FZD5,C2orf31,Frizzled-5,Fz-5,hFz5,FzE5宿主来源
Rabbit抗原名称
Fzd5免疫原
Synthetic Peptide细胞定位
Synapse, Cell membrane, Golgi apparatus membraneAccession
Q13467克隆号
S-1511-106抗体类型
Recombinant mAb抗体同种型
IgG应用
WB反应种属 ?
Hu阳性样本
HepG2, HEK-293, LoVo, HeLa, Jurkat, A549纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu |
Fzd5, also known as Frizzled-5, is a member of the G-protein coupled receptor Fz/Smo family, which plays a significant role in the Wnt signaling pathway. It is involved in tissue morphogenesis and cellular polarity information transmission. Fzd5 specifically interacts with Wnt5A to activate the beta-catenin pathway and is implicated in various cellular processes including proliferation, DNA damage repair, and stemness maintenance. It also contributes to chromatin accessibility regulation in intestinal epithelial cells, influencing stem cell fate and lineage generation. Furthermore, Fzd5 has been shown to be essential for the activity of alveolar epithelial stem cells and can be targeted to promote regeneration after lung injury. Research has also highlighted its role in cancer growth, particularly in pancreatic ductal adenocarcinoma (PDAC), where it mediates Wnt/β-catenin signaling, which is necessary for tumor survival and growth. Fzd5's function is intricately linked to cholesterol metabolism, as it can bind cholesterol, affecting its maturation and transport to the cell membrane, and this interaction is crucial for its signaling role.
免疫印迹
WB result of Fzd5 Recombinant Rabbit mAb
Primary antibody: Fzd5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: LoVo whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 65 kDa
Observed MW: 50~65 kDa
This blot was developed with high sensitivity substrate
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