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CHMP2B Recombinant Rabbit mAb (S-1639-20)

Charged multivesicular body protein 2b,CHMP2.5,Chromatin-modifying protein 2b (CHMP2b),Vacuolar protein sorting-associated protein 2-2 (Vps2-2,hVps2-2)

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1244

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

CHMP2B
分子别名

Charged multivesicular body protein 2b; CHMP2.5; Chromatin-modifying protein 2b (CHMP2b); Vacuolar protein sorting-associated protein 2-2 (Vps2-2; hVps2-2)
免疫原

Synthetic Peptide
细胞定位

Cytoplasm
Accession

Q9UQN3
克隆号

S-1639-20
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

HeLa, A549, A431, 293T, HepG2, mouse brain, rat brain
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ?

WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:500 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:500	Hu

背景介绍

CHMP2B is a component of the ESCRT-III complex, which plays a critical role in membrane remodeling and the regulation of endosomal trafficking, particularly in the degradation of endosomes into lysosomes. It is associated with the cytoskeleton in neurons and is thought to strengthen dendritic spines. Mutations in the CHMP2B gene are linked to autosomal dominant frontal temporal dementia (FTD) in humans, and are associated with the death of cortical neurons due to dendritic retraction and the accumulation of ubiquitinated proteins and autophagosomes. CHMP2B also has a role in regulating TDP-43 phosphorylation and cytotoxicity independent of autophagy via the protein kinase CK1. Furthermore, CHMP2B's function in the autophagy pathway and its impact on TDP-43 phosphorylation can independently contribute to cell toxicity and neurodegeneration. The protein's role in membrane remodeling is highlighted by its ability to form a protein network on the membrane surface, enhancing membrane rigidity, which is important for understanding the mechanics of membrane remodeling and scission.

免疫印迹
- WB result of CHMP2B Recombinant Rabbit mAb
  Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HeLa whole cell lysate 20 µg
  Lane 2: A549 whole cell lysate 20 µg
  Lane 3: A431 whole cell lysate 20 µg
  Lane 4: 293T whole cell lysate 20 µg
  Lane 5: HepG2 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 24 kDa
  Observed MW: 27 kDa
- WB result of CHMP2B Recombinant Rabbit mAb
  Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 24 kDa
  Observed MW: 27 kDa
- WB result of CHMP2B Recombinant Rabbit mAb
  Primary antibody: CHMP2B Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat brain lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 24 kDa
  Observed MW: 27 kDa
免疫细胞化学
- ICC shows positive staining in HepG2 cells. Anti- CHMP2B antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).