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Rabbit抗原名称
HIRA分子别名
Protein HIRA; TUP1-like enhancer of split protein 1; Tuple1免疫原
Recombinant Protein细胞定位
NucleusAccession
Q61666克隆号
S-1802-37抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
HEK-293, HeLa, K562, F9纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms
HIRA, or the Histone chaperone HIRA complex, is a crucial protein complex involved in chromatin remodeling and the regulation of gene expression. It plays a significant role in the deposition of the histone variant H3.3 into nucleosomes, which is essential for DNA replication, repair, and the maintenance of genomic integrity. HIRA is particularly important in embryonic stem cells, where it helps to control the activity of retrotransposons, thereby safeguarding the stability of the genome. Its function in these processes underscores the importance of HIRA in development and the prevention of diseases associated with genomic instability.
免疫印迹
WB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 112 kDaWB result of HIRA Recombinant Rabbit mAb
Primary antibody: HIRA Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 112 kDa
Observed MW: 112 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变,不同客户在回收抗体的保存条件上也会有差异,所以抗体回收使用效果无法保证。另外,我们对一批抗体回收验证测试,测试结果显示不同抗体可回收次数不同,一般效价越高的抗体,可重复使用的次数越多,客户可根据实验情况来确定







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