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Rabbit抗原名称
IFIT1分子别名
Interferon-induced protein with tetratricopeptide repeats 1; IFIT-1; Interferon-induced 56 kDa protein (IFI-56K; P56); G10P1; IFI56; IFNAI1; ISG56免疫原
Synthetic Peptide细胞定位
CytoplasmAccession
P09914克隆号
S-962-58抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)CyMk纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu IHC-P 1:500 Hu, Ms, Rt
IFIT1 (Interferon-induced protein with tetratricopeptide repeats 1) is a member of the ISG (Interferon-stimulated genes) family, which is induced upon interferon treatment or viral infection. Primarily located in the cytoplasm, IFIT1 contains multiple tetratricopeptide repeat (TPR) motifs that facilitate protein-protein interactions. It inhibits viral infection by recognizing and binding to viral RNAs that lack the 2'-O-methylated cap structure or possess a 5'-triphosphate group, thereby suppressing viral translation or sequestering them. Additionally, IFIT1 interacts with the eIF3e subunit of the eukaryotic translation initiation factor 3, inhibiting translation, and has been shown to suppress the replication of various RNA viruses.
免疫印迹
WB result of IFIT1 Recombinant Rabbit mAb
Primary antibody: IFIT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 10 ng/ml hIFN-α for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-IFIT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of IFIT1 in tumor tissue.
Expression of IFIT1 in human tissue.
Expression of IFIT1 in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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