热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 抗体 其他一抗

所有图片（1/2）

Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb (S-1489-86)

Low-density lipoprotein receptor-related protein 6,LRP-6

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B1241
规格
数量
收藏 分享
产品介绍

产品介绍 FAQs 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    Phospho-LRP6 (Ser1490)

  • 分子别名

    Low-density lipoprotein receptor-related protein 6; LRP-6

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    O75581

  • 克隆号

    S-1489-86

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 翻译后修饰类型

    磷酸化

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    WB

  • 稀释度

    应用 稀释度 推荐种属
    Dot Blot 1:1000
    WB 1:1000 Hu
背景介绍

  • Phospho-LRP6 (Ser1490) is a key phosphorylation site on the LRP6 protein, which is a component of the Wnt-Fzd-LRP5-LRP6 complex that initiates the β-catenin signaling pathway. This phosphorylation event is crucial for the aggregation of receptor-ligand complexes into ribosome-sized signalsomes, which are essential for the activation of the Wnt/β-catenin signaling. The phosphorylation of LRP6 at Ser1490, mediated by kinases such as GSK3 and CK1, is required for the binding of AXIN1 and subsequent stabilization and activation of β-catenin by preventing GSK3-mediated phosphorylation of β-catenin. This process is pivotal in various biological processes, including bone formation and embryonic development. The Phospho-LRP6 (Ser1490) antibody specifically detects endogenous levels of LRP6 only when it is phosphorylated at Ser1490, indicating the importance of this modification in the regulation of Wnt signaling.

  • 免疫印迹

    • WB result of Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb
      Primary antibody: Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: 293T transfected with empty vector whole cell lysate 20 µg
      Lane 2: 293T transfected with a construct expressing myc-His-tagged full-length LRP6 fusion protein whole cell lysate 20 µg
      Lane 3: 293T transfected with a construct expressing myc-His-tagged full-length LRP6 fusion protein, then treated with 100 ng/ml Wnt-3a for 15 minutes whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 180 kDa
      Observed MW: 200 kDa

  • 斑点杂交

    • Dot blot result of Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb
      Lane 1: LRP6 (Ser1490) phospho peptide
      Lane 2: LRP6 (Ser1490) phospho peptide
      Lane 3: LRP6 WT peptide
      Primary antibody: Phospho-LRP6 (Ser1490) Recombinant Rabbit mAb at 1/1000 dilution
      Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

检测磷酸化蛋白时，推荐有什么封闭体系？

我们推荐客户使用TPST+5%脱脂奶粉来稀释一抗，进行封闭。 虽然BSA被推荐为WB检测磷酸化蛋白的常用封闭剂，但是脱脂奶粉获取更加方便，覆盖更广泛的非特异性结合位点，在一抗性能优越的前提下，使用脱脂奶粉封闭性价比更高

评论(0)