大包装询价 产品介绍 评论(0)
宿主来源
Rabbit抗原名称
MMP2分子别名
72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2 (MMP-2); TBE-1; CLG4A免疫原
Recombinant Protein细胞定位
Nucleus, Secreted, CytoplasmAccession
P08253克隆号
S-1804-55抗体类型
Recombinant mAb抗体同种型
IgG应用
IHC-P, WB反应种属 ?
Hu, Ms阳性样本
U-87 MG, A375, NIH/3T3纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20°C as supplied
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu |
MMP2, also known as gelatinase A or 72 kDa type IV collagenase, is a matrix metalloproteinase that plays a crucial role in the degradation of the extracellular matrix (ECM), particularly type IV collagen, which is a major component of basement membranes. It is involved in multiple physiological processes such as angiogenesis, tissue remodeling, and repair. MMP2 also contributes to the breakdown of non-helical collagen and other proteins like fibronectin, laminin, natural insoluble elastin, aggrecan, and vitronectin. The enzyme is expressed by various cell types, including fibroblasts, keratinocytes, endothelial cells, chondrocytes, and monocytes. Furthermore, MMP2 has been implicated in the pathogenesis of several diseases, including atherosclerosis and skeletal disorders, and it plays a role in the angiogenesis associated with various diseases by releasing angiogenic factors and assisting in cell migration and adhesion. Understanding the pathological and physiological aspects of MMP2 is essential for developing therapeutic interventions for diseases where it plays a significant role.
免疫印迹
WB result of MMP2 Recombinant Rabbit mAb
Primary antibody: MMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: A375 whole cell lysate 20 µg
Negative control: HCT 116 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 74 kDa
Observed MW: 70 kDaWB result of MMP2 Recombinant Rabbit mAb
Primary antibody: MMP2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 74 kDa
Observed MW: 70 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-MMP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-MMP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-MMP2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of MMP2 in tumor tissue.
Expression of MMP2 in human tissue.
Expression of MMP2 in mouse & rat tissue.
评论(0)