产品中心 蛋白翻译后修饰(PTM)抗体 位点特异性修饰抗体
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宿主来源
Rabbit抗原名称
α-tubulin (acetyl K40)免疫原
Synthetic Peptide细胞定位
CytoskeletonAccession
P68366克隆号
S-1536-100抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
乙酰化反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Bv, Ck, Cz, Hm, Pg, Xe纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 推荐种属 Dot Blot 1:1000 WB 1:1000 Hu, Ms, Rt IP 1:50 IHC-P 1:500 Hu, Ms, Rt
α-Tubulin acetylation at lysine 40 (α-tubulin K40ac) is a post-translational modification that marks long-lived microtubules and plays a role in various cellular functions. This acetylation is associated with the stabilization of microtubules, particularly in structures like axons and cilia. It is believed to influence microtubule dynamics, potentially by affecting lateral interactions among protofilaments and increasing microtubule stability. α-Tubulin K40 acetylation is also implicated in intracellular transport, cell division, and cell migration, as well as in neuronal development, including axonal transport, neuronal migration, and axonal growth. Furthermore, it has been suggested that acetylated microtubules facilitate the transport of the Hippo regulator Merlin, which is crucial for contact inhibition of proliferation and cell-substrate adhesion. The modification is regulated by the acetyltransferase αTAT1 and deacetylases like HDAC6 and SIRT2, and it serves as a stable microtubule marker, enhancing the binding of motor proteins and playing a role in early polarization events in neurons. Deficiency in α-tubulin acetylation has been linked to impaired axonal transport and neurodegenerative disorders. Thus, α-tubulin (acetyl K40) is critical for maintaining the structural integrity of microtubules and regulating key cellular processes.
免疫印迹
WB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 400 nM TSA for 16 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
Observed MW: 55 kDaWB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
Observed MW: 55 kDaWB result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 500 ng/ml TSA for 4 hours whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50 kDa
Observed MW: 55 kDa
免疫沉淀
Acetyl-α-Tubulin (Lys40) Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Acetyl-α-Tubulin (Lys40) in 0.4 mg Mouse brain whole cell lysate.
Western blot was performed on the immunoprecipitate using Acetyl-α-Tubulin (Lys40) Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: Mouse brain whole cell lysate 20 µg (Input)
Lane 2: Acetyl-α-Tubulin (Lys40) Rabbit mAb IP in Mouse brain whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in Mouse brain whole cell lysate
Predicted MW: 50kDa
Observed MW: 55kDa
斑点杂交
Dot blot result of α-tubulin (acetyl K40) Recombinant Rabbit mAb
Lane 1: α-tubulin K40 acetyl peptide
Lane 2: α-tubulin K40 acetyl peptide
Lane 3: α-tubulin K40 unmodified peptide
Primary antibody: α-tubulin (acetyl K40) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
免疫组化
IHC shows positive staining in paraffin-embedded human kidney. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-α-tubulin (acetyl K40) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression ofα-tubulin (acetyl K40) in tumor tissue.
Expression ofα-tubulin (acetyl K40) in human tissue.
Expression ofα-tubulin (acetyl K40) in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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