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宿主来源
Rat抗原名称
Mouse CD4分子别名
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P06332克隆号
S-1259-5抗体类型
Rat mAb抗体同种型
IgG1,k应用
FCM反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
| 应用 | 稀释度 | 推荐种属 |
|---|---|---|
| FCM | 1:200 | Ms |
CD4 is a glycoprotein found on the surface of immune cells, primarily known for its role as a coreceptor on T-helper cells, a subset of T lymphocytes. CD4 is used as a marker to distinguish between different T cell subsets, particularly T-helper cells (CD4+ T cells) and T-regulatory cells (also known as Tregs, which can be CD4+CD25+). CD4 acts as a coreceptor with the T cell receptor (TCR) to recognize antigens presented on the surface of antigen-presenting cells (APCs) by MHC class II molecules. This interaction is crucial for the activation of T-helper cells. CD4+ T cells are central to the regulation of immune responses. They help activate B cells, macrophages, and other immune cells, and they produce cytokines that influence the type of immune response (e.g., TH1, TH2, Tfh, Tregs).
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD4 antibody at 1/200 (1 μg) dilution/ (Right panel) compared with a Rat IgG1 Isotype Control / (Left panel). Goat Anti-Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD8α - Alexa Fluor® 647 antibody separately.
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