Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb (S-1127-19)

DNA-binding factor KBF1,EBP-1,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B1233

规格

25μl100μl1ml

数量

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产品规格

宿主来源

Rabbit
抗原名称

Phospho-NF-kB p105/p50 (Ser337)
分子别名

DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
免疫原

Synthetic Peptide
细胞定位

Nucleus, Cytoplasm
Accession

P19838
克隆号

S-1127-19
抗体类型

Recombinant mAb
抗体同种型

IgG
翻译后修饰类型

磷酸化
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Ck, Dg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

WB
稀释度

应用稀释度推荐种属

Dot Blot 1:1000
WB 1：1000-1：5000 Hu, Ms, Rt

应用	稀释度	推荐种属
Dot Blot	1:1000
WB	1：1000-1：5000	Hu, Ms, Rt

背景介绍

Phospho-NF-kB p105/p50 (Ser337) is a phosphorylated form of the NF-kB subunits p105 and p50, which are crucial in the regulation of gene expression, particularly in response to various stimuli such as inflammation, immunity, differentiation, cell growth, and tumorigenesis. Phosphorylation at Ser337 is significant for the transcriptional activity of these subunits. It has been shown that phosphorylation at this site can affect the dimerization of p50 and its interaction with DNA. Specifically, phosphorylation by protein kinases like PKAc and Chk1 influences the ability of p50 to form homodimers and bind to DNA, which in turn modulates the transcriptional output of genes under the control of NF-kB. This phosphorylation event is also implicated in the gene-specific regulation of transcription, offering opportunities for selective targeting of NF-kB for therapeutic benefits. Thus, Phospho-NF-kB p105/p50 (Ser337) is a critical regulatory point in the NF-kB signaling pathway, impacting the transcriptional program that governs key physiological processes and disease states.

免疫印迹
- WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated HeLa whole cell lysate 20 µg
  Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
  Observed MW: 50, 110 kDa
- WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
  Lane 2: NIH/3T3 treated with 20 ng/ml TNF-alpha and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
  Observed MW: 55 kDa
- WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
  Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
  Lane 1: untreated C6 whole cell lysate 20 µg
  Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
  Observed MW: 60, 110 kDa
斑点杂交
- Dot blot result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
  Lane 1: NF-kB p105/p50 (Ser337) phospho peptide
  Lane 2: NF-kB p105/p50 unmodified peptide
  Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/1000 dilution
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution